Application: Special staining methods (toluidine blue staining, hematoxylin staining and flash freezing of sections with eosin) Tissue sections up to 20 µm Bone, frozen heart, brain, skin and other tissue sections that are usually difficult to mount Immunocytochemistry and DNA hybridization of frozen tissue sections Selective counting for immunofluorescence analysis in case of small amounts of tissue Superfrost® Plus Gold slides have special adhesives for fixing cells and tissues. No complex and expensive application of adhesives or protein layers is necessary. No unwanted background colors appear. Freshly frozen sections fixed in formalin are attracted to the slide and then chemically fixed to the glass surface.